Latest preprints
Colomb-F, Jamwal-A, Ogunkanbi-A, Frangova-T, Savage-AR, Kelly-S, Wright-GJ, Higgins-MK, McSorley-HJ Heparan sulphate binding controls in vivo half-life of the HpARI protein family Biorxiv (2024) doi: https://doi.org/10.1101/2024.05.06.592731
2024
Wang-LT, Cooper-AJR, Farrell-B, Miura-K, Diouf-A, Müller-Sienerth-N, Crosnier-C, Purser-L, Kirtley-PJ, Maciuszek-M, Barrett-JR, McHugh-K, Tucker-C, Li-S, Doumbo-S, Doumtabe-D, Pyo-CW, Nielsen-CM, Silk-SE, Kayentao-K, Ongoiba-A, Zhao-M, Nguyen-DC, Lee-FE, Minassian-AM, Geraghty-DE, Traore-B, Seder-RA, Wilder-BK, Crompton-PD, Wright-GJ, Long-CA, Draper-SJ, Higgins-MK, Tan-J Natural malaria infection elicits rare but potent neutralizing antibodies to the blood-stage antigen RH5. Cell doi: 10.1016/j.cell.2024.06.037 (2024).
Powell, G. T., Faro, A., Zhao, Y., Stickney, H., Novellasdemunt, L., Henriques, P., Gestri, G., White, E. R., Ren, J., Lu, W., Young, R. M., Hawkins, T. A., Cavodeassi, F., Schwarz, Q., Dreosti, E., Raible, D. W., Li, V. S. W., Wright, G. J., Yvonne Jones, E. & Wilson, S. W. Cachd1 interacts with Wnt receptors and regulates neuronal assymetry in the zebrafish brain. Science 384 p573 (2024).
Elofsson, A., Han, L,, Bianchi, E., Wright, G.J. & Jovine L. Deep learning insights into the architecture of the mammalian egg-sperm fusion synapse. Elife. 26 RP93131 (2024)
Shilts, J., Wright. G.J. Mapping the human cell surface interactome: a key to decode cell-to-cell communication. Annu Rev Biomed Data Sci. 2024 May 9. doi: 10.1146/annurev-biodatasci-102523-103821.
Segireddy, R. R., Belda, H., Yang, A.S.P., Dundas, K., Knoeckel, J., Galaway, F., Wood, L., Quinkert, D., Knuepfer, E., Treeck, M., Wright, G. J. & Douglas, A. D. A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions. Malaria J. (2024) 23(1):151. doi: 10.1186/s12936-024-04913-2
Roberts A.J., Ong H.B., Clare S., Brandt C., Harcourt K., Takele Y., Ghosh P., Toepp A., Waugh M., Matano D., Färnert A., Adams E., Moreno J., Mbuchi M., Petersen C., Mondal D., Kropf P. & Wright G.J. A panel of recombinant Leishmania donovani cell surface and secreted proteins identifies LdBPK_323600.1 as a serological marker of symptomatic infection. mBio. (2024)
Bianchi, E., Jiménez-Movilla, M., Cots-Rodríguez, P., Viola, C. & Wright, G. J. No evidence for a direct extracellular interaction between human Fc receptor-like 3 (MAIA) and the sperm ligand IZUMO1. Sci Adv 10, eadk6352 (2024).
Lee, S.-K., Crosnier, C., Valenzuela-Leon, P. C., Dizon, B. L. P., Atkinson, J. P., Mu, J., Wright, G. J., Calvo, E., Gunalan, K. & Miller, L. H. Complement receptor 1 is the human erythrocyte receptor for Plasmodium vivax erythrocyte binding protein. Proc. Natl. Acad. Sci. U. S. A. 121, e2316304121 (2024).
Powell, G. T., Faro, A., Zhao, Y., Stickney, H., Novellasdemunt, L., Henriques, P., Gestri, G., White, E. R., Ren, J., Lu, W., Young, R. M., Hawkins, T. A., Cavodeassi, F., Schwarz, Q., Dreosti, E., Raible, D. W., Li, V. S. W., Wright, G. J., Yvonne Jones, E. & Wilson, S. W. Cachd1 interacts with Wnt receptors and regulates neuronal assymetry in the zebrafish brain. Science 384 p573 (2024).
Elofsson, A., Han, L,, Bianchi, E., Wright, G.J. & Jovine L. Deep learning insights into the architecture of the mammalian egg-sperm fusion synapse. Elife. 26 RP93131 (2024)
Shilts, J., Wright. G.J. Mapping the human cell surface interactome: a key to decode cell-to-cell communication. Annu Rev Biomed Data Sci. 2024 May 9. doi: 10.1146/annurev-biodatasci-102523-103821.
Segireddy, R. R., Belda, H., Yang, A.S.P., Dundas, K., Knoeckel, J., Galaway, F., Wood, L., Quinkert, D., Knuepfer, E., Treeck, M., Wright, G. J. & Douglas, A. D. A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions. Malaria J. (2024) 23(1):151. doi: 10.1186/s12936-024-04913-2
Roberts A.J., Ong H.B., Clare S., Brandt C., Harcourt K., Takele Y., Ghosh P., Toepp A., Waugh M., Matano D., Färnert A., Adams E., Moreno J., Mbuchi M., Petersen C., Mondal D., Kropf P. & Wright G.J. A panel of recombinant Leishmania donovani cell surface and secreted proteins identifies LdBPK_323600.1 as a serological marker of symptomatic infection. mBio. (2024)
Bianchi, E., Jiménez-Movilla, M., Cots-Rodríguez, P., Viola, C. & Wright, G. J. No evidence for a direct extracellular interaction between human Fc receptor-like 3 (MAIA) and the sperm ligand IZUMO1. Sci Adv 10, eadk6352 (2024).
Lee, S.-K., Crosnier, C., Valenzuela-Leon, P. C., Dizon, B. L. P., Atkinson, J. P., Mu, J., Wright, G. J., Calvo, E., Gunalan, K. & Miller, L. H. Complement receptor 1 is the human erythrocyte receptor for Plasmodium vivax erythrocyte binding protein. Proc. Natl. Acad. Sci. U. S. A. 121, e2316304121 (2024).
2023
Hart, M. N., Mohring, F., Donvito, S. M., Thomas, J. A., Muller-Sienerth, N., Wright, G. J., Knuepfer, E., Saibil, H. R. & Moon, R. W. Sequential roles for red blood cell binding proteins enable phased commitment to invasion for malaria parasites. Nature Communications 2023 14 (2023).
Arutyunyan A, Roberts K, Troulé K, Wong FCK, Sheridan MA, Kats I, Garcia-Alonso L, Velten B, Hoo R, Ruiz-Morales ER, Sancho-Serra C, Shilts J, Handfield LF, Marconato L, Tuck E, Gardner L, Mazzeo CI, Li Q, Kelava I, Wright GJ, Prigmore E, Teichmann SA, Bayraktar OA, Moffett A, Stegle O, Turco MY, Vento-Tormo R. Spatial multiomics map of trophoblast development in early pregnancy. Nature. 616 p143 (2023).
Shilts, J., Crozier, T. W. M., Teixeira-Silva, A., Gabaev, I., Gerber, P. P., Greenwood, E. J. D., Watson, S. J., Ortmann, B. M., Gawden-Bone, C. M., Pauzaite, T., Hoffmann, M., Nathan, J. A., Pöhlmann, S., Matheson, N. J., Lehner, P. J. & Wright, G. J. LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike protein. PLoS Biol. 21, e3001959 (2023).
Bianchi, E. & Wright, G. J. Mammalian fertilization: Does sperm IZUMO1 mediate fusion as well as adhesion? J. Cell Biol. 222, (2023).
Arutyunyan A, Roberts K, Troulé K, Wong FCK, Sheridan MA, Kats I, Garcia-Alonso L, Velten B, Hoo R, Ruiz-Morales ER, Sancho-Serra C, Shilts J, Handfield LF, Marconato L, Tuck E, Gardner L, Mazzeo CI, Li Q, Kelava I, Wright GJ, Prigmore E, Teichmann SA, Bayraktar OA, Moffett A, Stegle O, Turco MY, Vento-Tormo R. Spatial multiomics map of trophoblast development in early pregnancy. Nature. 616 p143 (2023).
Shilts, J., Crozier, T. W. M., Teixeira-Silva, A., Gabaev, I., Gerber, P. P., Greenwood, E. J. D., Watson, S. J., Ortmann, B. M., Gawden-Bone, C. M., Pauzaite, T., Hoffmann, M., Nathan, J. A., Pöhlmann, S., Matheson, N. J., Lehner, P. J. & Wright, G. J. LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike protein. PLoS Biol. 21, e3001959 (2023).
Bianchi, E. & Wright, G. J. Mammalian fertilization: Does sperm IZUMO1 mediate fusion as well as adhesion? J. Cell Biol. 222, (2023).
2022
Shilts, J., Severin, Y., Galaway, F., Müller-Sienerth, N., Chong, Z.-S., Pritchard, S., Teichmann, S., Vento-Tormo, R., Snijder, B. & Wright, G. J. A physical wiring diagram for the human immune system. Nature (2022). doi:10.1038/s41586-022-05028-x
Romero-Ramirez, A., Casas-Sánchez, A., Autheman, D., Duffy, C. W., Brandt, C., Clare, S., Harcourt, K., André, M. R., de Almeida Castilho Neto, K. J. G., Teixeira, M. M. G., Machado, R. Z., Coombes, J., Flynn, R. J., Wright, G. J. & Jackson, A. P. Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface. PLoS Negl. Trop. Dis. 16, e0010791 (2022).
Cable, J., Rappuoli, R., Klemm, E. J., Kang, G., Mutreja, A., Wright, G. J., Pizza, M., Castro, S. A., Hoffmann, J. P., Alter, G., Carfi, A., Pollard, A. J., Krammer, F., Gupta, R. K., Wagner, C. E., Machado, V., Modjarrad, K., Corey, L., B Gilbert, P., Dougan, G., Lurie, N., Bjorkman, P. J., Chiu, C., Nemes, E., Gordon, S. B., Steer, A. C., Rudel, T., Blish, C. A., Sandberg, J. T., Brennan, K., Klugman, K. P., Stuart, L. M., Madhi, S. A. & Karp, C. L. Innovative vaccine approaches-a Keystone Symposia report. Ann. N. Y. Acad. Sci. (2022). doi:10.1111/nyas.14739
Roberts, A., Nagar, R., Brandt, C., Harcourt, K., Clare, S., Ferguson, M. A. J. & Wright, G. J. The Leishmania donovani Ortholog of the Glycosylphosphatidylinositol Anchor Biosynthesis Cofactor PBN1 Is Essential for Host Infection. MBio e0043322 (2022).
Stepanenko, N., Wolk, O., Bianchi, E., Wright, G. J., Schachter-Safrai, N., Makedonski, K., Ouro, A., Ben-Meir, A., Buganim, Y. & Goldblum, A. In silico Docking Analysis for Blocking JUNO‐IZUMO1 Interaction Identifies Two Small Molecules that Block in vitro Fertilization. Frontiers in Cell and Developmental Biology 10, (2022).
Roberts, A. J., Ong, H. B., Clare, S., Brandt, C., Harcourt, K., Franssen, S. U., Cotton, J. A., Müller-Sienerth, N. & Wright, G. J. Systematic identification of genes encoding cell surface and secreted proteins that are essential for in vitro growth and infection in Leishmania donovani. PLoS Pathog. 18, e1010364 (2022).
Romero-Ramirez, A., Casas-Sánchez, A., Autheman, D., Duffy, C. W., Brandt, C., Clare, S., Harcourt, K., André, M. R., de Almeida Castilho Neto, K. J. G., Teixeira, M. M. G., Machado, R. Z., Coombes, J., Flynn, R. J., Wright, G. J. & Jackson, A. P. Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface. PLoS Negl. Trop. Dis. 16, e0010791 (2022).
Cable, J., Rappuoli, R., Klemm, E. J., Kang, G., Mutreja, A., Wright, G. J., Pizza, M., Castro, S. A., Hoffmann, J. P., Alter, G., Carfi, A., Pollard, A. J., Krammer, F., Gupta, R. K., Wagner, C. E., Machado, V., Modjarrad, K., Corey, L., B Gilbert, P., Dougan, G., Lurie, N., Bjorkman, P. J., Chiu, C., Nemes, E., Gordon, S. B., Steer, A. C., Rudel, T., Blish, C. A., Sandberg, J. T., Brennan, K., Klugman, K. P., Stuart, L. M., Madhi, S. A. & Karp, C. L. Innovative vaccine approaches-a Keystone Symposia report. Ann. N. Y. Acad. Sci. (2022). doi:10.1111/nyas.14739
Roberts, A., Nagar, R., Brandt, C., Harcourt, K., Clare, S., Ferguson, M. A. J. & Wright, G. J. The Leishmania donovani Ortholog of the Glycosylphosphatidylinositol Anchor Biosynthesis Cofactor PBN1 Is Essential for Host Infection. MBio e0043322 (2022).
Stepanenko, N., Wolk, O., Bianchi, E., Wright, G. J., Schachter-Safrai, N., Makedonski, K., Ouro, A., Ben-Meir, A., Buganim, Y. & Goldblum, A. In silico Docking Analysis for Blocking JUNO‐IZUMO1 Interaction Identifies Two Small Molecules that Block in vitro Fertilization. Frontiers in Cell and Developmental Biology 10, (2022).
Roberts, A. J., Ong, H. B., Clare, S., Brandt, C., Harcourt, K., Franssen, S. U., Cotton, J. A., Müller-Sienerth, N. & Wright, G. J. Systematic identification of genes encoding cell surface and secreted proteins that are essential for in vitro growth and infection in Leishmania donovani. PLoS Pathog. 18, e1010364 (2022).
2021
Anisul, M., Shilts, J., Schwartzentruber, J., Hayhurst, J., Buniello, A., Shaikho Elhaj Mohammed, E., Zheng, J., Holmes, M., Ochoa, D., Carmona, M., Maranville, J., Gaunt, T. R., Emilsson, V., Gudnason, V., McDonagh, E. M., Wright, G. J., Ghoussaini, M. & Dunham, I. A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevance. Elife 10, (2021).
Autheman, D., Crosnier, C., Clare, S., Goulding, D. A., Brandt, C., Harcourt, K., Tolley, C., Galaway, F., Khushu, M., Ong, H., Romero-Ramirez, A., Duffy, C. W., Jackson, A. P. & Wright, G. J. An invariant Trypanosoma vivax vaccine antigen induces protective immunity. Nature (2021). doi:10.1038/s41586-021-03597-x
Bianchi, E., Sun, Y., Almansa-Ordonez, A., Woods, M., Goulding, D., Martinez-Martin, N. & Wright, G. J. Control of oviductal fluid flow by the G-protein coupled receptor Adgrd1 is essential for murine embryo transit. Nature Communications 12, (2021).
Knöckel, J., Dundas, K., Yang, A. S. P., Galaway, F., Metcalf, T., van Gemert, G.-J., Sauerwein, R. W., Rayner, J. C., Billker, O. & Wright, G. J. Systematic Identification of Plasmodium Falciparum Sporozoite Membrane Protein Interactions Reveals an Essential Role for the p24 Complex in Host Infection. Molecular & Cellular Proteomics 20, 100038 (2021).
Shilts, J., Crozier, T. W. M., Greenwood, E. J. D., Lehner, P. J. & Wright, G. J. No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor. Sci. Rep. 11, 413 (2021).
Awuah-Mensah, G., McDonald, J., Steketee, P. C., Autheman, D., Whipple, S., D’Archivio, S., Brandt, C., Clare, S., Harcourt, K., Wright, G. J., Morrison, L. J., Gadelha, C. & Wickstead, B. Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo. PLoS Pathog. 17, e1009224 (2021).
Autheman, D., Crosnier, C., Clare, S., Goulding, D. A., Brandt, C., Harcourt, K., Tolley, C., Galaway, F., Khushu, M., Ong, H., Romero-Ramirez, A., Duffy, C. W., Jackson, A. P. & Wright, G. J. An invariant Trypanosoma vivax vaccine antigen induces protective immunity. Nature (2021). doi:10.1038/s41586-021-03597-x
Bianchi, E., Sun, Y., Almansa-Ordonez, A., Woods, M., Goulding, D., Martinez-Martin, N. & Wright, G. J. Control of oviductal fluid flow by the G-protein coupled receptor Adgrd1 is essential for murine embryo transit. Nature Communications 12, (2021).
Knöckel, J., Dundas, K., Yang, A. S. P., Galaway, F., Metcalf, T., van Gemert, G.-J., Sauerwein, R. W., Rayner, J. C., Billker, O. & Wright, G. J. Systematic Identification of Plasmodium Falciparum Sporozoite Membrane Protein Interactions Reveals an Essential Role for the p24 Complex in Host Infection. Molecular & Cellular Proteomics 20, 100038 (2021).
Shilts, J., Crozier, T. W. M., Greenwood, E. J. D., Lehner, P. J. & Wright, G. J. No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor. Sci. Rep. 11, 413 (2021).
Awuah-Mensah, G., McDonald, J., Steketee, P. C., Autheman, D., Whipple, S., D’Archivio, S., Brandt, C., Clare, S., Harcourt, K., Wright, G. J., Morrison, L. J., Gadelha, C. & Wickstead, B. Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo. PLoS Pathog. 17, e1009224 (2021).
2020
Sharma, S., Dincer, C., Weidemüller, P., Wright, G. J. & Petsalaki, E. CEN-tools: an integrative platform to identify the contexts of essential genes. Mol. Syst. Biol. 16, e9698 (2020).
Campeotto, I., Galaway, F., Mehmood, S., Barfod, L. K., Quinkert, D., Kotraiah, V., Phares, T. W., Wright, K. E., Snijders, A. P., Draper, S. J., Higgins, M. K. & Wright, G. J. The Structure of the Cysteine-Rich Domain of Plasmodium falciparum P113 Identifies the Location of the RH5 Binding Site. MBio 11, (2020).
Crosnier, C., Hokke, C. H., Protasio, A. V., Brandt, C., Rinaldi, G., Langenberg, M. C. C., Clare, S., Janse, J. J., Wilson, S., Berriman, M., Roestenberg, M. & Wright, G. J. Screening of a library of recombinant Schistosoma mansoni proteins with sera from murine and human controlled infections identifies early serological markers. J. Infect. Dis. (2020). doi:10.1093/infdis/jiaa329
Galaway, F. & Wright, G. J. Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase. Sci. Rep. 10, 10522 (2020).
Lamas-Toranzo, I., Hamze, J. G., Bianchi, E., Fernández-Fuertes, B., Pérez-Cerezales, S., Laguna-Barraza, R., Fernández-González, R., Lonergan, P., Gutiérrez-Adán, A., Wright, G. J., Jiménez-Movilla, M. & Bermejo-Álvarez, P. TMEM95 is a sperm membrane protein essential for mammalian fertilization. Elife 9, (2020).
Ong, H. B., Clare, S., Roberts, A. J., Wilson, M. E. & Wright, G. J. Establishment, optimisation and quantitation of a bioluminescent murine infection model of visceral leishmaniasis for systematic vaccine screening. Sci. Rep. 10, 4689 (2020).
Sharma, S. & Wright, G. J. Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens. J. Vis. Exp. (2020). doi:10.3791/60803
Müller-Sienerth, N., Shilts, J., Kadir, K. A., Yman, V., Homann, M. V., Asghar, M., Ngasala, B., Singh, B., Färnert, A. & Wright, G. J. A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance. Malar. J. 19, 31 (2020).
Segireddy, R. R., Dundas, K., Knoeckel, J., Galaway, F., Wood, L., Wright, G. J. & Douglas, A. D. A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions. bioRxiv 2020.02.02.929190 (2020). doi:10.1101/2020.02.02.929190
Campeotto, I., Galaway, F., Mehmood, S., Barfod, L. K., Quinkert, D., Kotraiah, V., Phares, T. W., Wright, K. E., Snijders, A. P., Draper, S. J., Higgins, M. K. & Wright, G. J. The Structure of the Cysteine-Rich Domain of Plasmodium falciparum P113 Identifies the Location of the RH5 Binding Site. MBio 11, (2020).
Crosnier, C., Hokke, C. H., Protasio, A. V., Brandt, C., Rinaldi, G., Langenberg, M. C. C., Clare, S., Janse, J. J., Wilson, S., Berriman, M., Roestenberg, M. & Wright, G. J. Screening of a library of recombinant Schistosoma mansoni proteins with sera from murine and human controlled infections identifies early serological markers. J. Infect. Dis. (2020). doi:10.1093/infdis/jiaa329
Galaway, F. & Wright, G. J. Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase. Sci. Rep. 10, 10522 (2020).
Lamas-Toranzo, I., Hamze, J. G., Bianchi, E., Fernández-Fuertes, B., Pérez-Cerezales, S., Laguna-Barraza, R., Fernández-González, R., Lonergan, P., Gutiérrez-Adán, A., Wright, G. J., Jiménez-Movilla, M. & Bermejo-Álvarez, P. TMEM95 is a sperm membrane protein essential for mammalian fertilization. Elife 9, (2020).
Ong, H. B., Clare, S., Roberts, A. J., Wilson, M. E. & Wright, G. J. Establishment, optimisation and quantitation of a bioluminescent murine infection model of visceral leishmaniasis for systematic vaccine screening. Sci. Rep. 10, 4689 (2020).
Sharma, S. & Wright, G. J. Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens. J. Vis. Exp. (2020). doi:10.3791/60803
Müller-Sienerth, N., Shilts, J., Kadir, K. A., Yman, V., Homann, M. V., Asghar, M., Ngasala, B., Singh, B., Färnert, A. & Wright, G. J. A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance. Malar. J. 19, 31 (2020).
Segireddy, R. R., Dundas, K., Knoeckel, J., Galaway, F., Wood, L., Wright, G. J. & Douglas, A. D. A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions. bioRxiv 2020.02.02.929190 (2020). doi:10.1101/2020.02.02.929190
2019
Elton, C. M., Rodriguez, M., Ben Mamoun, C., Lobo, C. A. & Wright, G. J. A library of recombinant Babesia microti cell surface and secreted proteins for diagnostics discovery and reverse vaccinology. Int. J. Parasitol. 49, 115–125 (2019).
Crosnier, C., Brandt, C., Rinaldi, G., McCarthy, C., Barker, C., Clare, S., Berriman, M. & Wright, G. J. Systematic screening of 96 Schistosoma mansoni cell-surface and secreted antigens does not identify any strongly protective vaccine candidates in a mouse model of infection. Wellcome Open Res 4, 159 (2019).
Collins, C., Lui, Y., Santos, A. M., Ballif, B. A., Gogerly-Moragoda, A. M., Brouwer, H., Ross, R., Balagurunathan, K., Sharma, S., Wright, G. J., Davis, S. & Budd, R. C. Detection of Cell Surface Ligands for Human Synovial γδ T Cells. J. Immunol. 203, 2369–2376 (2019).
Wood, L. & Wright, G. J. High-Content Imaging for Large-Scale Detection of Low-Affinity Extracellular Protein Interactions. SLAS Discov 2472555219879053 (2019).
Galaway, F., Yu, R., Constantinou, A., Prugnolle, F. & Wright, G. J. Resurrection of the ancestral RH5 invasion ligand provides a molecular explanation for the origin of P. falciparum malaria in humans. PLoS Biol. 17, e3000490 (2019).
Proto, W. R., Siegel, S. V., Dankwa, S., Liu, W., Kemp, A., Marsden, S., Zenonos, Z. A., Unwin, S., Sharp, P. M., Wright, G. J., Hahn, B. H., Duraisingh, M. T. & Rayner, J. C. Adaptation of Plasmodium falciparum to humans involved the loss of an ape-specific erythrocyte invasion ligand. Nat. Commun. 10, 4512 (2019).
Vögtle, T., Sharma, S., Mori, J., Nagy, Z., Semeniak, D., Scandola, C., Geer, M. J., Smith, C. W., Lane, J., Pollack, S., Lassila, R., Jouppila, A., Barr, A. J., Ogg, D. J., Howard, T. D., McMiken, H. J., Warwicker, J., Geh, C., Rowlinson, R., Abbott, W. M., Eckly, A., Schulze, H., Wright, G. J., Mazharian, A., Fütterer, K., Rajesh, S., Douglas, M. R. & Senis, Y. A. Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B. Elife 8, (2019).
Mensah-Brown, H. E., Aspeling-Jones, H., Delimini, R. K., Asante, K. P., Amlabu, E., Bah, S. Y., Beeson, J. G., Wright, G. J., Conway, D. J. & Awandare, G. A. Antibody Reactivity to Merozoite Antigens in Ghanaian Adults Correlates With Growth Inhibitory Activity Against Plasmodium falciparum in Culture. Open Forum Infect Dis 6, ofz254 (2019).
Alanine, D. G. W., Quinkert, D., Kumarasingha, R., Mehmood, S., Donnellan, F. R., Minkah, N. K., Dadonaite, B., Diouf, A., Galaway, F., Silk, S. E., Jamwal, A., Marshall, J. M., Miura, K., Foquet, L., Elias, S. C., Labbé, G. M., Douglas, A. D., Jin, J., Payne, R. O., Illingworth, J. J., Pattinson, D. J., Pulido, D., Williams, B. G., de Jongh, W. A., Wright, G. J., Kappe, S. H. I., Robinson, C. V., Long, C. A., Crabb, B. S., Gilson, P. R., Higgins, M. K. & Draper, S. J. Human Antibodies that Slow Erythrocyte Invasion Potentiate Malaria-Neutralizing Antibodies. Cell 178, 216–228.e21 (2019).
Douglas, A. D., Baldeviano, G. C., Jin, J., Miura, K., Diouf, A., Zenonos, Z. A., Ventocilla, J. A., Silk, S. E., Marshall, J. M., Alanine, D. G. W., Wang, C., Edwards, N. J., Leiva, K. P., Gomez-Puerta, L. A., Lucas, C. M., Wright, G. J., Long, C. A., Royal, J. M. & Draper, S. J. A defined mechanistic correlate of protection against Plasmodium falciparum malaria in non-human primates. Nat. Commun. 10, 1953 (2019).
Crosnier, C., Brandt, C., Rinaldi, G., McCarthy, C., Barker, C., Clare, S., Berriman, M. & Wright, G. J. Systematic screening of 96 Schistosoma mansoni cell-surface and secreted antigens does not identify any strongly protective vaccine candidates in a mouse model of infection. Wellcome Open Res 4, 159 (2019).
Collins, C., Lui, Y., Santos, A. M., Ballif, B. A., Gogerly-Moragoda, A. M., Brouwer, H., Ross, R., Balagurunathan, K., Sharma, S., Wright, G. J., Davis, S. & Budd, R. C. Detection of Cell Surface Ligands for Human Synovial γδ T Cells. J. Immunol. 203, 2369–2376 (2019).
Wood, L. & Wright, G. J. High-Content Imaging for Large-Scale Detection of Low-Affinity Extracellular Protein Interactions. SLAS Discov 2472555219879053 (2019).
Galaway, F., Yu, R., Constantinou, A., Prugnolle, F. & Wright, G. J. Resurrection of the ancestral RH5 invasion ligand provides a molecular explanation for the origin of P. falciparum malaria in humans. PLoS Biol. 17, e3000490 (2019).
Proto, W. R., Siegel, S. V., Dankwa, S., Liu, W., Kemp, A., Marsden, S., Zenonos, Z. A., Unwin, S., Sharp, P. M., Wright, G. J., Hahn, B. H., Duraisingh, M. T. & Rayner, J. C. Adaptation of Plasmodium falciparum to humans involved the loss of an ape-specific erythrocyte invasion ligand. Nat. Commun. 10, 4512 (2019).
Vögtle, T., Sharma, S., Mori, J., Nagy, Z., Semeniak, D., Scandola, C., Geer, M. J., Smith, C. W., Lane, J., Pollack, S., Lassila, R., Jouppila, A., Barr, A. J., Ogg, D. J., Howard, T. D., McMiken, H. J., Warwicker, J., Geh, C., Rowlinson, R., Abbott, W. M., Eckly, A., Schulze, H., Wright, G. J., Mazharian, A., Fütterer, K., Rajesh, S., Douglas, M. R. & Senis, Y. A. Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B. Elife 8, (2019).
Mensah-Brown, H. E., Aspeling-Jones, H., Delimini, R. K., Asante, K. P., Amlabu, E., Bah, S. Y., Beeson, J. G., Wright, G. J., Conway, D. J. & Awandare, G. A. Antibody Reactivity to Merozoite Antigens in Ghanaian Adults Correlates With Growth Inhibitory Activity Against Plasmodium falciparum in Culture. Open Forum Infect Dis 6, ofz254 (2019).
Alanine, D. G. W., Quinkert, D., Kumarasingha, R., Mehmood, S., Donnellan, F. R., Minkah, N. K., Dadonaite, B., Diouf, A., Galaway, F., Silk, S. E., Jamwal, A., Marshall, J. M., Miura, K., Foquet, L., Elias, S. C., Labbé, G. M., Douglas, A. D., Jin, J., Payne, R. O., Illingworth, J. J., Pattinson, D. J., Pulido, D., Williams, B. G., de Jongh, W. A., Wright, G. J., Kappe, S. H. I., Robinson, C. V., Long, C. A., Crabb, B. S., Gilson, P. R., Higgins, M. K. & Draper, S. J. Human Antibodies that Slow Erythrocyte Invasion Potentiate Malaria-Neutralizing Antibodies. Cell 178, 216–228.e21 (2019).
Douglas, A. D., Baldeviano, G. C., Jin, J., Miura, K., Diouf, A., Zenonos, Z. A., Ventocilla, J. A., Silk, S. E., Marshall, J. M., Alanine, D. G. W., Wang, C., Edwards, N. J., Leiva, K. P., Gomez-Puerta, L. A., Lucas, C. M., Wright, G. J., Long, C. A., Royal, J. M. & Draper, S. J. A defined mechanistic correlate of protection against Plasmodium falciparum malaria in non-human primates. Nat. Commun. 10, 1953 (2019).
2018
Sharma, S., Bartholdson, S. J., Couch, A. C. M., Yusa, K. & Wright, G. J. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res. 28, 1372–1382 (2018).
Dundas, K., Shears, M. J., Sun, Y., Hopp, C. S., Crosnier, C., Metcalf, T., Girling, G., Sinnis, P., Billker, O. & Wright, G. J. Alpha-v-containing integrins are host receptors for the Plasmodium falciparum sporozoite surface protein, TRAP. Proc. Natl. Acad. Sci. U. S. A. 115, 4477–4482 (2018).
Yi, W., Bao, W., Rodriguez, M., Liu, Y., Singh, M., Ramlall, V., Cursino-Santos, J. R., Zhong, H., Elton, C. M., Wright, G. J., Mendelson, A., An, X., Lobo, C. A. & Yazdanbakhsh, K. Robust adaptive immune response against Babesia microti infection marked by low parasitemia in a murine model of sickle cell disease. Blood Adv 2, 3462–3478 (2018).
Chong, Z.-S., Ohnishi, S., Yusa, K. & Wright, G. J. Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 19, 205 (2018).
Vento-Tormo, R., Efremova, M., Botting, R. A., Turco, M. Y., Vento-Tormo, M., Meyer, K. B., Park, J.-E., Stephenson, E., Polański, K., Goncalves, A., Gardner, L., Holmqvist, S., Henriksson, J., Zou, A., Sharkey, A. M., Millar, B., Innes, B., Wood, L., Wilbrey-Clark, A., Payne, R. P., Ivarsson, M. A., Lisgo, S., Filby, A., Rowitch, D. H., Bulmer, J. N., Wright, G. J., Stubbington, M. J. T., Haniffa, M., Moffett, A. & Teichmann, S. A. Single-cell reconstruction of the early maternal-fetal interface in humans. Nature 563, 347–353 (2018).
Dundas, K., Shears, M. J., Sun, Y., Hopp, C. S., Crosnier, C., Metcalf, T., Girling, G., Sinnis, P., Billker, O. & Wright, G. J. Alpha-v-containing integrins are host receptors for the Plasmodium falciparum sporozoite surface protein, TRAP. Proc. Natl. Acad. Sci. U. S. A. 115, 4477–4482 (2018).
Yi, W., Bao, W., Rodriguez, M., Liu, Y., Singh, M., Ramlall, V., Cursino-Santos, J. R., Zhong, H., Elton, C. M., Wright, G. J., Mendelson, A., An, X., Lobo, C. A. & Yazdanbakhsh, K. Robust adaptive immune response against Babesia microti infection marked by low parasitemia in a murine model of sickle cell disease. Blood Adv 2, 3462–3478 (2018).
Chong, Z.-S., Ohnishi, S., Yusa, K. & Wright, G. J. Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome Biol. 19, 205 (2018).
Vento-Tormo, R., Efremova, M., Botting, R. A., Turco, M. Y., Vento-Tormo, M., Meyer, K. B., Park, J.-E., Stephenson, E., Polański, K., Goncalves, A., Gardner, L., Holmqvist, S., Henriksson, J., Zou, A., Sharkey, A. M., Millar, B., Innes, B., Wood, L., Wilbrey-Clark, A., Payne, R. P., Ivarsson, M. A., Lisgo, S., Filby, A., Rowitch, D. H., Bulmer, J. N., Wright, G. J., Stubbington, M. J. T., Haniffa, M., Moffett, A. & Teichmann, S. A. Single-cell reconstruction of the early maternal-fetal interface in humans. Nature 563, 347–353 (2018).
2017
Payne, R. O., Silk, S. E., Elias, S. C., Miura, K., Diouf, A., Galaway, F., de Graaf, H., Brendish, N. J., Poulton, I. D., Griffiths, O. J., Edwards, N. J., Jin, J., Labbé, G. M., Alanine, D. G., Siani, L., Di Marco, S., Roberts, R., Green, N., Berrie, E., Ishizuka, A. S., Nielsen, C. M., Bardelli, M., Partey, F. D., Ofori, M. F., Barfod, L., Wambua, J., Murungi, L. M., Osier, F. H., Biswas, S., McCarthy, J. S., Minassian, A. M., Ashfield, R., Viebig, N. K., Nugent, F. L., Douglas, A. D., Vekemans, J., Wright, G. J., Faust, S. N., Hill, A. V., Long, C. A., Lawrie, A. M. & Draper, S. J. Human vaccination against RH5 induces neutralizing antimalarial antibodies that inhibit RH5 invasion complex interactions. JCI Insight 2, (2017).
Bustamante, L. Y., Powell, G. T., Lin, Y.-C., Macklin, M. D., Cross, N., Kemp, A., Cawkill, P., Sanderson, T., Crosnier, C., Muller-Sienerth, N., Doumbo, O. K., Traore, B., Crompton, P. D., Cicuta, P., Tran, T. M., Wright, G. J. & Rayner, J. C. Synergistic malaria vaccine combinations identified by systematic antigen screening. Proc. Natl. Acad. Sci. U. S. A. 114, 12045–12050 (2017).
Galaway, F., Drought, L. G., Fala, M., Cross, N., Kemp, A. C., Rayner, J. C. & Wright, G. J. P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5. Nat. Commun. 8, 14333 (2017).
Bustamante, L. Y., Powell, G. T., Lin, Y.-C., Macklin, M. D., Cross, N., Kemp, A., Cawkill, P., Sanderson, T., Crosnier, C., Muller-Sienerth, N., Doumbo, O. K., Traore, B., Crompton, P. D., Cicuta, P., Tran, T. M., Wright, G. J. & Rayner, J. C. Synergistic malaria vaccine combinations identified by systematic antigen screening. Proc. Natl. Acad. Sci. U. S. A. 114, 12045–12050 (2017).
Galaway, F., Drought, L. G., Fala, M., Cross, N., Kemp, A. C., Rayner, J. C. & Wright, G. J. P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5. Nat. Commun. 8, 14333 (2017).
2016
Cayuso, J., Dzementsei, A., Fischer, J. C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G. J. & Ober, E. A. EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality. Dev. Cell 39, 316–328 (2016).
Nishimura, K., Han, L., Bianchi, E., Wright, G. J., de Sanctis, D. & Jovine, L. The structure of sperm Izumo1 reveals unexpected similarities with Plasmodium invasion proteins. Curr. Biol. 26, R661–2 (2016).
Crosnier, C., Iqbal, Z., Knuepfer, E., Maciuca, S., Perrin, A. J., Kamuyu, G., Goulding, D., Bustamante, L. Y., Miles, A., Moore, S. C., Dougan, G., Holder, A. A., Kwiatkowski, D. P., Rayner, J. C., Pleass, R. J. & Wright, G. J. Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants. J. Biol. Chem. 291, 14285–14299 (2016).
Han, L., Nishimura, K., Sadat Al Hosseini, H., Bianchi, E., Wright, G. J. & Jovine, L. Divergent evolution of vitamin B9 binding underlies Juno-mediated adhesion of mammalian gametes. Curr. Biol. 26, R100–1 (2016).
Nishimura, K., Han, L., Bianchi, E., Wright, G. J., de Sanctis, D. & Jovine, L. The structure of sperm Izumo1 reveals unexpected similarities with Plasmodium invasion proteins. Curr. Biol. 26, R661–2 (2016).
Crosnier, C., Iqbal, Z., Knuepfer, E., Maciuca, S., Perrin, A. J., Kamuyu, G., Goulding, D., Bustamante, L. Y., Miles, A., Moore, S. C., Dougan, G., Holder, A. A., Kwiatkowski, D. P., Rayner, J. C., Pleass, R. J. & Wright, G. J. Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants. J. Biol. Chem. 291, 14285–14299 (2016).
Han, L., Nishimura, K., Sadat Al Hosseini, H., Bianchi, E., Wright, G. J. & Jovine, L. Divergent evolution of vitamin B9 binding underlies Juno-mediated adhesion of mammalian gametes. Curr. Biol. 26, R100–1 (2016).
2015
Hostetler, J. B., Sharma, S., Bartholdson, S. J., Wright, G. J., Fairhurst, R. M. & Rayner, J. C. A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions. PLoS Negl. Trop. Dis. 9, e0004264 (2015).
Das, S., Hertrich, N., Perrin, A. J., Withers-Martinez, C., Collins, C. R., Jones, M. L., Watermeyer, J. M., Fobes, E. T., Martin, S. R., Saibil, H. R., Wright, G. J., Treeck, M., Epp, C. & Blackman, M. J. Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs. Cell Host Microbe 18, 433–444 (2015).
Panza, P., Sitko, A. A., Maischein, H.-M., Koch, I., Flötenmeyer, M., Wright, G. J., Mandai, K., Mason, C. A. & Söllner, C. The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm. Neural Dev. 10, 23 (2015).
Zenonos, Z. A., Dummler, S. K., Müller-Sienerth, N., Chen, J., Preiser, P. R., Rayner, J. C. & Wright, G. J. Basigin is a druggable target for host-oriented antimalarial interventions. J. Exp. Med. 212, 1145–1151 (2015).
Perrin, A. J., Bartholdson, S. J. & Wright, G. J. P-selectin is a host receptor for Plasmodium MSP7 ligands. Malar. J. 14, 238 (2015).
Sun, Y., Vandenbriele, C., Kauskot, A., Verhamme, P., Hoylaerts, M. F. & Wright, G. J. A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand. Mol. Cell. Proteomics 14, 1265–1274 (2015).
Douglas, A. D., Baldeviano, G. C., Lucas, C. M., Lugo-Roman, L. A., Crosnier, C., Bartholdson, S. J., Diouf, A., Miura, K., Lambert, L. E., Ventocilla, J. A., Leiva, K. P., Milne, K. H., Illingworth, J. J., Spencer, A. J., Hjerrild, K. A., Alanine, D. G. W., Turner, A. V., Moorhead, J. T., Edgel, K. A., Wu, Y., Long, C. A., Wright, G. J., Lescano, A. G. & Draper, S. J. A PfRH5-based vaccine is efficacious against heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys. Cell Host Microbe 17, 130–139 (2015).
Weiss, G. E., Gilson, P. R., Taechalertpaisarn, T., Tham, W.-H., de Jong, N. W. M., Harvey, K. L., Fowkes, F. J. I., Barlow, P. N., Rayner, J. C., Wright, G. J., Cowman, A. F. & Crabb, B. S. Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes. PLoS Pathog. 11, e1004670 (2015).
Saetear, P., Perrin, A. J., Bartholdson, S. J., Wanaguru, M., Kussrow, A., Bornhop, D. J. & Wright, G. J. Quantification of Plasmodium-host protein interactions on intact, unmodified erythrocytes by back-scattering interferometry. Malar. J. 14, 88 (2015).
Staudt, N., Müller-Sienerth, N., Fane-Dremucheva, A., Yusaf, S. P., Millrine, D. & Wright, G. J. A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining. Biochem. Biophys. Res. Commun. 456, 527–533 (2015).
Bianchi, E. & Wright, G. J. Cross-species fertilization: the hamster egg receptor, Juno, binds the human sperm ligand, Izumo1. Philos. Trans. R. Soc. Lond. B Biol. Sci. 370, 20140101 (2015).
Das, S., Hertrich, N., Perrin, A. J., Withers-Martinez, C., Collins, C. R., Jones, M. L., Watermeyer, J. M., Fobes, E. T., Martin, S. R., Saibil, H. R., Wright, G. J., Treeck, M., Epp, C. & Blackman, M. J. Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs. Cell Host Microbe 18, 433–444 (2015).
Panza, P., Sitko, A. A., Maischein, H.-M., Koch, I., Flötenmeyer, M., Wright, G. J., Mandai, K., Mason, C. A. & Söllner, C. The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm. Neural Dev. 10, 23 (2015).
Zenonos, Z. A., Dummler, S. K., Müller-Sienerth, N., Chen, J., Preiser, P. R., Rayner, J. C. & Wright, G. J. Basigin is a druggable target for host-oriented antimalarial interventions. J. Exp. Med. 212, 1145–1151 (2015).
Perrin, A. J., Bartholdson, S. J. & Wright, G. J. P-selectin is a host receptor for Plasmodium MSP7 ligands. Malar. J. 14, 238 (2015).
Sun, Y., Vandenbriele, C., Kauskot, A., Verhamme, P., Hoylaerts, M. F. & Wright, G. J. A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand. Mol. Cell. Proteomics 14, 1265–1274 (2015).
Douglas, A. D., Baldeviano, G. C., Lucas, C. M., Lugo-Roman, L. A., Crosnier, C., Bartholdson, S. J., Diouf, A., Miura, K., Lambert, L. E., Ventocilla, J. A., Leiva, K. P., Milne, K. H., Illingworth, J. J., Spencer, A. J., Hjerrild, K. A., Alanine, D. G. W., Turner, A. V., Moorhead, J. T., Edgel, K. A., Wu, Y., Long, C. A., Wright, G. J., Lescano, A. G. & Draper, S. J. A PfRH5-based vaccine is efficacious against heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys. Cell Host Microbe 17, 130–139 (2015).
Weiss, G. E., Gilson, P. R., Taechalertpaisarn, T., Tham, W.-H., de Jong, N. W. M., Harvey, K. L., Fowkes, F. J. I., Barlow, P. N., Rayner, J. C., Wright, G. J., Cowman, A. F. & Crabb, B. S. Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes. PLoS Pathog. 11, e1004670 (2015).
Saetear, P., Perrin, A. J., Bartholdson, S. J., Wanaguru, M., Kussrow, A., Bornhop, D. J. & Wright, G. J. Quantification of Plasmodium-host protein interactions on intact, unmodified erythrocytes by back-scattering interferometry. Malar. J. 14, 88 (2015).
Staudt, N., Müller-Sienerth, N., Fane-Dremucheva, A., Yusaf, S. P., Millrine, D. & Wright, G. J. A panel of recombinant monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining. Biochem. Biophys. Res. Commun. 456, 527–533 (2015).
Bianchi, E. & Wright, G. J. Cross-species fertilization: the hamster egg receptor, Juno, binds the human sperm ligand, Izumo1. Philos. Trans. R. Soc. Lond. B Biol. Sci. 370, 20140101 (2015).
2014
Douglas, A. D., Williams, A. R., Knuepfer, E., Illingworth, J. J., Furze, J. M., Crosnier, C., Choudhary, P., Bustamante, L. Y., Zakutansky, S. E., Awuah, D. K., Alanine, D. G. W., Theron, M., Worth, A., Shimkets, R., Rayner, J. C., Holder, A. A., Wright, G. J. & Draper, S. J. Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5. J. Immunol. 192, 245–258 (2014).
Tran, T. M., Ongoiba, A., Coursen, J., Crosnier, C., Diouf, A., Huang, C.-Y., Li, S., Doumbo, S., Doumtabe, D., Kone, Y., Bathily, A., Dia, S., Niangaly, M., Dara, C., Sangala, J., Miller, L. H., Doumbo, O. K., Kayentao, K., Long, C. A., Miura, K., Wright, G. J., Traore, B. & Crompton, P. D. Naturally acquired antibodies specific for Plasmodium falciparum reticulocyte-binding protein homologue 5 inhibit parasite growth and predict protection from malaria. J. Infect. Dis. 209, 789–798 (2014).
Osier, F. H., Mackinnon, M. J., Crosnier, C., Fegan, G., Kamuyu, G., Wanaguru, M., Ogada, E., McDade, B., Rayner, J. C., Wright, G. J. & Marsh, K. New antigens for a multicomponent blood-stage malaria vaccine. Sci. Transl. Med. 6, 247ra102 (2014).
Bianchi, E., Doe, B., Goulding, D. & Wright, G. J. Juno is the egg Izumo receptor and is essential for mammalian fertilization. Nature 508, 483–487 (2014).
Zenonos, Z. A., Rayner, J. C. & Wright, G. J. Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library. Malar. J. 13, 93 (2014).
Staudt, N., Müller-Sienerth, N. & Wright, G. J. Development of an antigen microarray for high throughput monoclonal antibody selection. Biochem. Biophys. Res. Commun. 445, 785–790 (2014).
Tran, T. M., Ongoiba, A., Coursen, J., Crosnier, C., Diouf, A., Huang, C.-Y., Li, S., Doumbo, S., Doumtabe, D., Kone, Y., Bathily, A., Dia, S., Niangaly, M., Dara, C., Sangala, J., Miller, L. H., Doumbo, O. K., Kayentao, K., Long, C. A., Miura, K., Wright, G. J., Traore, B. & Crompton, P. D. Naturally acquired antibodies specific for Plasmodium falciparum reticulocyte-binding protein homologue 5 inhibit parasite growth and predict protection from malaria. J. Infect. Dis. 209, 789–798 (2014).
Osier, F. H., Mackinnon, M. J., Crosnier, C., Fegan, G., Kamuyu, G., Wanaguru, M., Ogada, E., McDade, B., Rayner, J. C., Wright, G. J. & Marsh, K. New antigens for a multicomponent blood-stage malaria vaccine. Sci. Transl. Med. 6, 247ra102 (2014).
Bianchi, E., Doe, B., Goulding, D. & Wright, G. J. Juno is the egg Izumo receptor and is essential for mammalian fertilization. Nature 508, 483–487 (2014).
Zenonos, Z. A., Rayner, J. C. & Wright, G. J. Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library. Malar. J. 13, 93 (2014).
Staudt, N., Müller-Sienerth, N. & Wright, G. J. Development of an antigen microarray for high throughput monoclonal antibody selection. Biochem. Biophys. Res. Commun. 445, 785–790 (2014).
2013
Wanaguru, M., Liu, W., Hahn, B. H., Rayner, J. C. & Wright, G. J. RH5-Basigin interaction plays a major role in the host tropism of Plasmodium falciparum. Proc. Natl. Acad. Sci. U. S. A. 110, 20735–20740 (2013).
Buysse, K., Riemersma, M., Powell, G., van Reeuwijk, J., Chitayat, D., Roscioli, T., Kamsteeg, E.-J., van den Elzen, C., van Beusekom, E., Blaser, S., Babul-Hirji, R., Halliday, W., Wright, G. J., Stemple, D. L., Lin, Y.-Y., Lefeber, D. J. & van Bokhoven, H. Missense mutations in β-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) cause Walker-Warburg syndrome. Hum. Mol. Genet. 22, 1746–1754 (2013).
Lane-Serff, H., Sun, Y., Metcalfe, P. & Wright, G. J. Expression of recombinant ITGA2 and CD109 for the detection of human platelet antigen (HPA)-5 and -15 alloantibodies. Br. J. Haematol. 161, 453–455 (2013).
Bustamante, L. Y., Bartholdson, S. J., Crosnier, C., Campos, M. G., Wanaguru, M., Nguon, C., Kwiatkowski, D. P., Wright, G. J. & Rayner, J. C. A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants. Vaccine 31, 373–379 (2013).
Crosnier, C., Wanaguru, M., McDade, B., Osier, F. H., Marsh, K., Rayner, J. C. & Wright, G. J. A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins. Mol. Cell. Proteomics 12, 3976–3986 (2013).
Wanaguru, M., Crosnier, C., Johnson, S., Rayner, J. C. & Wright, G. J. Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design. J. Biol. Chem. 288, 32106–32117 (2013).
Buysse, K., Riemersma, M., Powell, G., van Reeuwijk, J., Chitayat, D., Roscioli, T., Kamsteeg, E.-J., van den Elzen, C., van Beusekom, E., Blaser, S., Babul-Hirji, R., Halliday, W., Wright, G. J., Stemple, D. L., Lin, Y.-Y., Lefeber, D. J. & van Bokhoven, H. Missense mutations in β-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) cause Walker-Warburg syndrome. Hum. Mol. Genet. 22, 1746–1754 (2013).
Lane-Serff, H., Sun, Y., Metcalfe, P. & Wright, G. J. Expression of recombinant ITGA2 and CD109 for the detection of human platelet antigen (HPA)-5 and -15 alloantibodies. Br. J. Haematol. 161, 453–455 (2013).
Bustamante, L. Y., Bartholdson, S. J., Crosnier, C., Campos, M. G., Wanaguru, M., Nguon, C., Kwiatkowski, D. P., Wright, G. J. & Rayner, J. C. A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants. Vaccine 31, 373–379 (2013).
Crosnier, C., Wanaguru, M., McDade, B., Osier, F. H., Marsh, K., Rayner, J. C. & Wright, G. J. A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins. Mol. Cell. Proteomics 12, 3976–3986 (2013).
Wanaguru, M., Crosnier, C., Johnson, S., Rayner, J. C. & Wright, G. J. Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design. J. Biol. Chem. 288, 32106–32117 (2013).
2012
Bartholdson, S. J., Bustamante, L. Y., Crosnier, C., Johnson, S., Lea, S., Rayner, J. C. & Wright, G. J. Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specific TRAP homolog, MTRAP. PLoS Pathog. 8, e1003031 (2012).
Williams, A. R., Douglas, A. D., Miura, K., Illingworth, J. J., Choudhary, P., Murungi, L. M., Furze, J. M., Diouf, A., Miotto, O., Crosnier, C., Wright, G. J., Kwiatkowski, D. P., Fairhurst, R. M., Long, C. A. & Draper, S. J. Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens. PLoS Pathog. 8, e1002991 (2012).
Taechalertpaisarn, T., Crosnier, C., Bartholdson, S. J., Hodder, A. N., Thompson, J., Bustamante, L. Y., Wilson, D. W., Sanders, P. R., Wright, G. J., Rayner, J. C., Cowman, A. F., Gilson, P. R. & Crabb, B. S. Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41. PLoS One 7, e41937 (2012).
Powell, G. T. & Wright, G. J. Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. PLoS One 7, e40810 (2012).
Kerr, J. S. & Wright, G. J. Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions. J. Vis. Exp. e3881 (2012).
Sun, Y., Gallagher-Jones, M., Barker, C. & Wright, G. J. A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions. Anal. Biochem. 424, 45–53 (2012).
Williams, A. R., Douglas, A. D., Miura, K., Illingworth, J. J., Choudhary, P., Murungi, L. M., Furze, J. M., Diouf, A., Miotto, O., Crosnier, C., Wright, G. J., Kwiatkowski, D. P., Fairhurst, R. M., Long, C. A. & Draper, S. J. Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens. PLoS Pathog. 8, e1002991 (2012).
Taechalertpaisarn, T., Crosnier, C., Bartholdson, S. J., Hodder, A. N., Thompson, J., Bustamante, L. Y., Wilson, D. W., Sanders, P. R., Wright, G. J., Rayner, J. C., Cowman, A. F., Gilson, P. R. & Crabb, B. S. Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41. PLoS One 7, e41937 (2012).
Powell, G. T. & Wright, G. J. Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. PLoS One 7, e40810 (2012).
Kerr, J. S. & Wright, G. J. Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions. J. Vis. Exp. e3881 (2012).
Sun, Y., Gallagher-Jones, M., Barker, C. & Wright, G. J. A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions. Anal. Biochem. 424, 45–53 (2012).
2011
Douglas, A. D., Williams, A. R., Illingworth, J. J., Kamuyu, G., Biswas, S., Goodman, A. L., Wyllie, D. H., Crosnier, C., Miura, K., Wright, G. J., Long, C. A., Osier, F. H., Marsh, K., Turner, A. V., Hill, A. V. S. & Draper, S. J. The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody. Nat. Commun. 2, 601 (2011).
Powell, G. T. & Wright, G. J. Jamb and jamc are essential for vertebrate myocyte fusion. PLoS Biol. 9, e1001216 (2011).
Crosnier, C., Bustamante, L. Y., Bartholdson, S. J., Bei, A. K., Theron, M., Uchikawa, M., Mboup, S., Ndir, O., Kwiatkowski, D. P., Duraisingh, M. T., Rayner, J. C. & Wright, G. J. Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature 480, 534–537 (2011).
Wright, G. J., Giudicelli, F., Soza-Ried, C., Hanisch, A., Ariza-McNaughton, L. & Lewis, J. DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock. Development 138, 2947–2956 (2011).
Powell, G. T. & Wright, G. J. Jamb and jamc are essential for vertebrate myocyte fusion. PLoS Biol. 9, e1001216 (2011).
Crosnier, C., Bustamante, L. Y., Bartholdson, S. J., Bei, A. K., Theron, M., Uchikawa, M., Mboup, S., Ndir, O., Kwiatkowski, D. P., Duraisingh, M. T., Rayner, J. C. & Wright, G. J. Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature 480, 534–537 (2011).
Wright, G. J., Giudicelli, F., Soza-Ried, C., Hanisch, A., Ariza-McNaughton, L. & Lewis, J. DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock. Development 138, 2947–2956 (2011).
Pre-2011
Charoensawan, V., Adryan, B., Martin, S., Söllner, C., Thisse, B., Thisse, C., Wright, G. J. & Teichmann, S. A. The impact of gene expression regulation on evolution of extracellular signaling pathways. Mol. Cell. Proteomics 9, 2666–2677 (2010).
Martin, S., Söllner, C., Charoensawan, V., Adryan, B., Thisse, B., Thisse, C., Teichmann, S. & Wright, G. J. Construction of a Large Extracellular Protein Interaction Network and Its Resolution by Spatiotemporal Expression Profiling. Mol. Cell. Proteomics 9, 2654–2665 (2010).
Crosnier, C., Staudt, N. & Wright, G. J. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biol. 8, 76 (2010).
O’Brien, G. S., Martin, S. M., Söllner, C., Wright, G. J., Becker, C. G., Portera-Cailliau, C. & Sagasti, A. Developmentally regulated impediments to skin reinnervation by injured peripheral sensory axon terminals. Curr. Biol. 19, 2086–2090 (2009).
Söllner, C. & Wright, G. J. A cell surface interaction network of neural leucine-rich repeat receptors. Genome Biol. 10, R99 (2009).
Bushell, K. M., Söllner, C., Schuster-Boeckler, B., Bateman, A. & Wright, G. J. Large-scale screening for novel low-affinity extracellular protein interactions. Genome Res. 18, 622–630 (2008).
Giudicelli, F., Ozbudak, E. M., Wright, G. J. & Lewis, J. Setting the tempo in development: an investigation of the zebrafish somite clock mechanism. PLoS Biol. 5, e150 (2007).
Wright, G. J., Leslie, J. D., Ariza-McNaughton, L. & Lewis, J. Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development. Development 131, 5659–5669 (2004).
Foster-Cuevas, M., Wright, G. J., Puklavec, M. J., Brown, M. H. & Barclay, A. N. Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J. Virol. 78, 7667–7676 (2004).
Wright, G. J., Cherwinski, H., Foster-Cuevas, M., Brooke, G., Puklavec, M. J., Bigler, M., Song, Y., Jenmalm, M., Gorman, D., McClanahan, T., Liu, M.-R., Brown, M. H., Sedgwick, J. D., Phillips, J. H. & Barclay, A. N. Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J. Immunol. 171, 3034–3046 (2003).
Itoh, M., Kim, C.-H., Palardy, G., Oda, T., Jiang, Y.-J., Maust, D., Yeo, S.-Y., Lorick, K., Wright, G. J., Ariza-McNaughton, L., Weissman, A. M., Lewis, J., Chandrasekharappa, S. C. & Chitnis, A. B. Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta. Dev. Cell 4, 67–82 (2003).
Wright, G. J., Jones, M., Puklavec, M. J., Brown, M. H. & Barclay, A. N. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology 102, 173–179 (2001).
85. Dick, A. D., Broderick, C., Forrester, J. V. & Wright, G. J. Distribution of OX2 antigen and OX2 receptor within retina. Invest. Ophthalmol. Vis. Sci. 42, 170–176 (2001).
Hoek, R. M., Ruuls, S. R., Murphy, C. A., Wright, G. J., Goddard, R., Zurawski, S. M., Blom, B., Homola, M. E., Streit, W. J., Brown, M. H., Barclay, A. N. & Sedgwick, J. D. Down-regulation of the macrophage lineage through interaction with OX2 (CD200). Science 290, 1768–1771 (2000).
Wright, G. J., Puklavec, M. J., Willis, A. C., Hoek, R. M., Sedgwick, J. D., Brown, M. H. & Barclay, A. N. Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity 13, 233–242 (2000).
Preston, S., Wright, G. J., Starr, K., Barclay, A. N. & Brown, M. H. The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages. Eur. J. Immunol. 27, 1911–1918 (1997).
Martin, S., Söllner, C., Charoensawan, V., Adryan, B., Thisse, B., Thisse, C., Teichmann, S. & Wright, G. J. Construction of a Large Extracellular Protein Interaction Network and Its Resolution by Spatiotemporal Expression Profiling. Mol. Cell. Proteomics 9, 2654–2665 (2010).
Crosnier, C., Staudt, N. & Wright, G. J. A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biol. 8, 76 (2010).
O’Brien, G. S., Martin, S. M., Söllner, C., Wright, G. J., Becker, C. G., Portera-Cailliau, C. & Sagasti, A. Developmentally regulated impediments to skin reinnervation by injured peripheral sensory axon terminals. Curr. Biol. 19, 2086–2090 (2009).
Söllner, C. & Wright, G. J. A cell surface interaction network of neural leucine-rich repeat receptors. Genome Biol. 10, R99 (2009).
Bushell, K. M., Söllner, C., Schuster-Boeckler, B., Bateman, A. & Wright, G. J. Large-scale screening for novel low-affinity extracellular protein interactions. Genome Res. 18, 622–630 (2008).
Giudicelli, F., Ozbudak, E. M., Wright, G. J. & Lewis, J. Setting the tempo in development: an investigation of the zebrafish somite clock mechanism. PLoS Biol. 5, e150 (2007).
Wright, G. J., Leslie, J. D., Ariza-McNaughton, L. & Lewis, J. Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development. Development 131, 5659–5669 (2004).
Foster-Cuevas, M., Wright, G. J., Puklavec, M. J., Brown, M. H. & Barclay, A. N. Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J. Virol. 78, 7667–7676 (2004).
Wright, G. J., Cherwinski, H., Foster-Cuevas, M., Brooke, G., Puklavec, M. J., Bigler, M., Song, Y., Jenmalm, M., Gorman, D., McClanahan, T., Liu, M.-R., Brown, M. H., Sedgwick, J. D., Phillips, J. H. & Barclay, A. N. Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J. Immunol. 171, 3034–3046 (2003).
Itoh, M., Kim, C.-H., Palardy, G., Oda, T., Jiang, Y.-J., Maust, D., Yeo, S.-Y., Lorick, K., Wright, G. J., Ariza-McNaughton, L., Weissman, A. M., Lewis, J., Chandrasekharappa, S. C. & Chitnis, A. B. Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta. Dev. Cell 4, 67–82 (2003).
Wright, G. J., Jones, M., Puklavec, M. J., Brown, M. H. & Barclay, A. N. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology 102, 173–179 (2001).
85. Dick, A. D., Broderick, C., Forrester, J. V. & Wright, G. J. Distribution of OX2 antigen and OX2 receptor within retina. Invest. Ophthalmol. Vis. Sci. 42, 170–176 (2001).
Hoek, R. M., Ruuls, S. R., Murphy, C. A., Wright, G. J., Goddard, R., Zurawski, S. M., Blom, B., Homola, M. E., Streit, W. J., Brown, M. H., Barclay, A. N. & Sedgwick, J. D. Down-regulation of the macrophage lineage through interaction with OX2 (CD200). Science 290, 1768–1771 (2000).
Wright, G. J., Puklavec, M. J., Willis, A. C., Hoek, R. M., Sedgwick, J. D., Brown, M. H. & Barclay, A. N. Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity 13, 233–242 (2000).
Preston, S., Wright, G. J., Starr, K., Barclay, A. N. & Brown, M. H. The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages. Eur. J. Immunol. 27, 1911–1918 (1997).
